The chromatography of growth hormone on cellulose derivatives.

In this paper a report is made of the chromatographic behavior of growth hormone on columns of carboxymethylcellulose and diethylaminoethylcellulose. Ion exchange chromatography on the synthetic resin, Amberlite IRC-50, has been utilized to purify and to assess the homogeneity of a number of proteins. This resin has been used in chromatographic studies on cytochrome c (l), ribonuclease (2), lysozyme (3), chymotrypsin o( (4), and sheep fetal CO hemoglobin and bovine hemoglobin (5). Recently, certain derivatives of cellulose possessing ion exchange groups have also been studied with regard to the fractionation of proteins. Peterson and Sober have published reports on the use of columns of carboxymethylcellulose and diethylaminoethylcellulose in the fractionation of plasma proteins (6, 7) and of enzymes (8). Although the details of the preparation of the adsorbents which they used (9) have become available since the present study was completed, a brief description of the preparation of cellulose derivatives is included in this paper. This is desirable, since differences exist in the conditions of preparation such as concentration, temperature, and reaction time, which may affect the adsorption properties of the resulting cellulose derivatives.